Journal: BMC infectious diseases

Article Title: Signature of chronic hepatitis B virus infection in nails and hair.

doi: 10.1186/s12879-022-07400-8

Figure Lengend Snippet: Fig. 1 A The correlation between serum HBV DNA and nail HBV DNA. No significant correlation was observed (n = 27, r = − 0.111, p = 0.582). B The correlation between serum HBV DNA and hair HBV DNA. No significant correlation was observed (n = 18, r = 0.285, p = 0.251). C The correlation between nail HBV DNA and hair HBV DNA. There was a weak but significant correlation between nail HBV DNA and hair HBV DNA (n = 48, r = 0.378, p < 0.01). D The correlation between nail HBV DNA and hair HBsAg. No significant correlation was observed (n = 34, r = 0.255, p = 0.145)

Article Snippet: The primary antibody for the detection of HBsAg and hepatitis D (HD) antigen in the fingernail was mouse anti-HBsAg monoclonal antibody (sc-57785, Santa Cruz Biotechnology, Santa Cruz, CA) at the concentration of 2 μL/mL and mouse antiHDV monoclonal antibody (MC406.3, Gentaur Molecular Products, Brussels, Belgium) at the concentration of 10 μL/mL, respectively.

Techniques:

Journal: BMC infectious diseases

Article Title: Signature of chronic hepatitis B virus infection in nails and hair.

doi: 10.1186/s12879-022-07400-8

Figure Lengend Snippet: Fig. 2 The changes of nail/hair HBV DNA and nail HBsAg level due to the antiviral treatment. Clinical courses of five patients are shown. A Male; 20 years old, B female; 24 years old, C female; 6 years old, D female; 20 years old

Article Snippet: The primary antibody for the detection of HBsAg and hepatitis D (HD) antigen in the fingernail was mouse anti-HBsAg monoclonal antibody (sc-57785, Santa Cruz Biotechnology, Santa Cruz, CA) at the concentration of 2 μL/mL and mouse antiHDV monoclonal antibody (MC406.3, Gentaur Molecular Products, Brussels, Belgium) at the concentration of 10 μL/mL, respectively.

Techniques:

Journal: BMC infectious diseases

Article Title: Signature of chronic hepatitis B virus infection in nails and hair.

doi: 10.1186/s12879-022-07400-8

Figure Lengend Snippet: Fig. 5 A, B Immunohistochemical staining of nail from a patient with chronic HBV mono-infection. Original magnification = ×200. A HBsAg is stained brown. B The negative controls (mouse IgG1). C, D Immunohistochemical staining of fingernail from a patient with chronic HBV and HDV dual infections (no. HDV-14). Original magnification = ×200. C HBsAg is stained brown. D Negative control (mouse IgG1). E, F Negative control. Immunohistochemical staining of fingernail from a serum HBsAg negative subject. Original magnification = ×200

Article Snippet: The primary antibody for the detection of HBsAg and hepatitis D (HD) antigen in the fingernail was mouse anti-HBsAg monoclonal antibody (sc-57785, Santa Cruz Biotechnology, Santa Cruz, CA) at the concentration of 2 μL/mL and mouse antiHDV monoclonal antibody (MC406.3, Gentaur Molecular Products, Brussels, Belgium) at the concentration of 10 μL/mL, respectively.

Techniques: Immunohistochemical staining, Staining, Infection, Negative Control

Journal: BMC infectious diseases

Article Title: Signature of chronic hepatitis B virus infection in nails and hair.

doi: 10.1186/s12879-022-07400-8

Figure Lengend Snippet: Fig. 6 Immunohistochemical staining of fingernail from a patient with chronic HBV and HDV dual infections (no. HDV-14). Original magnification = ×400. A Hepatitis D antigen was detected at the edge of the nail. C Hepatitis D antigen was detected inside of the nail. B, D are the negative controls (mouse IgG1). E, F Negative control. immunohistochemical staining of fingernail from a serum HBsAg negative subject. Original magnification = ×400

Article Snippet: The primary antibody for the detection of HBsAg and hepatitis D (HD) antigen in the fingernail was mouse anti-HBsAg monoclonal antibody (sc-57785, Santa Cruz Biotechnology, Santa Cruz, CA) at the concentration of 2 μL/mL and mouse antiHDV monoclonal antibody (MC406.3, Gentaur Molecular Products, Brussels, Belgium) at the concentration of 10 μL/mL, respectively.

Techniques: Immunohistochemical staining, Staining, Negative Control

Journal: Frontiers in Oncology

Article Title: Integrative Proteo-Genomic Analysis for Recurrent Survival Prognosis in Colon Adenocarcinoma

doi: 10.3389/fonc.2022.871568

Figure Lengend Snippet: The list of independent prognostic feature cDEGs.

Article Snippet: The membranes were blocked with 5% skim milk dissolved in phosphate-buffered saline (PBS) and Tween 20 (PBST) for 2 h at room temperature, followed by incubated with primary antibodies against CA12 (15180-1-AP, Proteintech Group, Inc., Chicago, Il, USA), AHNAK2 (ab164994, Abcam), MMAA (ab264418, Abcam), DMBT1 (ab276422), and GAPDH (10491-1-AP, Proteintech Group, Inc., Chicago, Il, USA) at 4°C overnight.

Techniques:

Journal: Frontiers in Oncology

Article Title: Integrative Proteo-Genomic Analysis for Recurrent Survival Prognosis in Colon Adenocarcinoma

doi: 10.3389/fonc.2022.871568

Figure Lengend Snippet: Validation of eight independent prognostic cDEGs. Quantitative real-time PCR analysis was performed to determine the mRNA expression levels of ADH4, AHNAK2, MMAA, and DMBT1 in tumor tissue and adjacent tissues (A) , and in recurrent tissues and non-recurrent tissues (B) . Western blot analysis was utilized to measure the protein levels of ADH4, AHNAK2, MMAA, and DMBT1 in tumor tissue and adjacent tissues (C) , and in recurrent tissues and non-recurrent tissues (D) . Data were expressed as mean ± SD of three independent repeats. ** p < 0.01, *** p < 0.001, compared with adjacent or non-recurrent tissues. A, adjacent tissues; T, tumor tissues; N, non-recurrent; R, recurrent.

Article Snippet: The membranes were blocked with 5% skim milk dissolved in phosphate-buffered saline (PBS) and Tween 20 (PBST) for 2 h at room temperature, followed by incubated with primary antibodies against CA12 (15180-1-AP, Proteintech Group, Inc., Chicago, Il, USA), AHNAK2 (ab164994, Abcam), MMAA (ab264418, Abcam), DMBT1 (ab276422), and GAPDH (10491-1-AP, Proteintech Group, Inc., Chicago, Il, USA) at 4°C overnight.

Techniques: Biomarker Discovery, Real-time Polymerase Chain Reaction, Expressing, Western Blot